This paper examines the effect of a naturally occurring wNoto Wolbachia strain on the vector competence of Aedes notoscriptus mosquitoes for dengue-2 virus. Dengue-2 virus infection and dissemination rates and viral titres in wNoto infected Ae. notoscriptus were not significantly different compared to those in mosquitoes from which Wolbachia had been removed. The low density and restricted tissue distribution of the wNoto infection may underlie the absence of Wolbachia-mediated viral interference in this system.